Apsilon

Apsilon

The Apsilon™ family of DNA/RNA extraction kits offers a fast, enzyme-free, alcohol-free, room-temperature-stable approach to nucleic acid purification across a sweeping range of sample types, including whole blood, swabs, tissues, fluids, and even challenging matrices like FFPE, stool, and soil. Designed for both manual and high-throughput automated workflows, Apsilon™ kits achieve extraction in 10–13 minutes without centrifugation, bead drying, or enzymatic pretreatment, yet deliver high yields and consistently strong performance in qPCR, RT-qPCR, LAMP, and NGS. With up to 90% recovery, minimal inhibition, and proven superiority or parity with leading commercial kits, the Apsilon™ system provides a single streamlined solution for pathogen detection, host nucleic acid extraction, and advanced sequencing applications — all supported by an ambient-temperature Molecular Transport Medium that inactivates pathogens while preserving DNA and RNA.

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Core Technology

  • Magnetic-bead extraction optimized for speed and purity.

  • No enzymes required (no Proteinase K, no lysozyme).

  • Alcohol-free formulation for safe shipping and consistent chemistry.

  • Room-temperature stability — no refrigeration or cold chain needed.

  • 10–13 minute extraction depending on kit.

  • Manual or automated processing (compatible with KingFisher, M32, AutoPure Mini, etc.)

  • No centrifugation, no bead drying, no pretreatment even for tough organisms.

Performance

  • Up to 90% nucleic acid recovery.

  • High-purity DNA/RNA with minimal PCR inhibition.

  • Strong performance validated against major competitors (Q, M, N, T, K kits).

  • Compatible with:

    • qPCR / RT-qPCR

    • Isothermal assays (LAMP/RT-LAMP)

    • NGS / metagenomic sequencing

  • Demonstrated ability to extract host DNA/RNA + microbial nucleic acids simultaneously.

Sample Type Coverage

Apsilon™ kits support an unusually broad set of matrices:

  • Whole blood, serum, plasma

  • Respiratory, genito, wound, vaginal, rectal swabs (dry or UTM/VTM/MTM)

  • Saliva, urine, stool

  • Tissues, FFPE, cell pellets, culture media

  • Nasal swabs

  • Purified exosomes

  • Soil and other complex environmental samples

  • Pathogens:

    • DNA/RNA viruses

    • Gram-positive & gram-negative bacteria

    • Mycobacteria

    • Fungi

    • Host genomic DNA / RNA

Kit Portfolio Overview

  • Universal DNA/RNA Extraction Kit

  • Whole Blood DNA/RNA Extraction Kit (Host & Pathogen)

  • Nasal Swab DNA/RNA Extraction Kit

  • STD (Swab) DNA/RNA Extraction Kit

  • Nasal Swab DNA-only Extraction Kit (in development)

  • Formats: 24-prep, 96-prep, 480-prep

Workflow Advantages

  • Simple 1-plate protocol for automation.

  • Full extraction in 10–13 minutes (system or manual).

  • Unified chemistry across sample types → simplified training & logistics.

  • No hazardous materials, enabling easier shipping & storage.

  • Reduced plastic usage (40% less) for lower environmental impact.

Molecular Transport Medium (Apsilon™ MTM)

  • Inactivates viruses within seconds and bacteria within minutes.

  • Preserves RNA (3 months) and DNA (18 months) at room temperature.

  • Ethanol-free and fully compatible with all Apsilon™ extraction kits.

  • Ensures safe sample transport without refrigeration.

  • Demonstrated broad compatibility with downstream assays and sequencing.

NGS-Focused Capabilities

  • Superior microbial diversity recovery vs leading kits (higher Shannon & Gini-Simpson indices).

  • Better detection of Gram-positive species and broader taxonomic coverage.

  • High Q20/Q30 sequencing quality

  • Suitable for metagenomics, 16S/ITS sequencing, and cfDNA/miRNA workflows.